研究者情報 | 神奈川大学

イワクラ　イズミ Iwakura Izumi 岩倉　いずみ所属神奈川大学化学生命学部応用化学科神奈川大学大学院工学研究科工学専攻（応用化学領域）職種教授
言語種別	英語
発行・発表の年月	2024/01
形態種別	学術雑誌
査読	査読あり
標題	Excited State Vibrational Dynamics Reveals a Photocycle That Enhances the Photostability of the TagRFP-T Fluorescent Protein
執筆形態	共著
掲載誌名	The Journal of Physical Chemistry B
掲載区分	国外
出版社・発行元	American Chemical Society
巻・号・頁	128(5),pp.1188-1193
国際共著	国際共著
著者・共著者	Atsushi Yabushita, Chia-Yun Cheng, Ying Kuan Ko, Takayoshi Kobayashi, Izumi Iwakura, Ralph Jimenez
概要	High photostability is a desirable property of fluorescent proteins (FPs) for imaging, yet its molecular basis is poorly understood. We performed ultrafast spectroscopy on TagRFP and its 9-fold more photostable variant TagRFP-T to characterize their initial photoreactions. We find significant differences in their electronic and vibrational dynamics, including faster excited-state proton transfer and transient changes in the frequency of the v520 mode in the excited electronic state of TagRFP-T. The frequency of v520, which is sensitive to chromophore planarity, downshifts within 0.58 ps and recovers within 0.87 ps. This vibrational mode modulates the distance from the chromophore phenoxy to the side chain of residue N143, which we suggest can trigger photoisomerization. In TagRFP, the dynamics of v520 is missing, and this FP therefore lacks an important channel for chromophore isomerization. These dynamics are likely to be a key mechanism differentiating the photostability of the FPs.
DOI	https://doi.org/10.1021/acs.jpcb.3c07212
researchmap用URL	https://pubs.acs.org/doi/10.1021/acs.jpcb.3c07212