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イノウエ カズヒト
Inoue Kazuhito 井上 和仁 所属 神奈川大学 化学生命学部 生命機能学科 神奈川大学大学院 理学研究科 理学専攻（生物科学領域） 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/09 |
| 形態種別 | 学術雑誌 |
| 査読 | 査読あり |
| 標題 | SoxAX binding protein, a novel component of the thiosulfate-oxidizing multienzyme system in the green sulfur bacterium Chlorobium tepidum |
| 執筆形態 | 共著 |
| 掲載誌名 | J. Bacteriol. |
| 出版社・発行元 | American society for Microbiology |
| 巻・号・頁 | 190,pp.6097-6110 |
| 著者・共著者 | ◎T. Ogawa, T. Furusawa,R. Nomura, D. Seo, N. M. Matsuda, H. Sakurai, K. Inoue |
| 概要 | From the photosynthetic green sulfur bacterium Chlorobium tepidum , we have purified three factors indispensable for the thiosulfate-dependent reduction of the small, monoheme cytochrome c554. The first factor is SoxYZ , the second factor is monomeric SoxB and the third factor is the trimeric cytochrome c551, composed of the monoheme cytochrome SoxA, the monoheme cytochrome SoxX, and the product of the hypothetical open reading frame CT1020. In the presence of the other two Sox factors, the recombinant SoxA and SoxX showed a low but discernible thiosulfate-dependent cytochrome c554 reduction activity. The further addition of the recombinant CT1020 protein greatly increased the activity, and the total activity was as high as that of the native SoxAX-CT1020 protein complex. The recombinant CT1020 protein participated in the formation of a tight complex with SoxA and SoxX and will be referred to as SAXB (SoxAX binding protein). |
| DOI | 10.1128/JB.00634-08 |
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