研究者情報 | 神奈川大学

イワクラ　イズミ Iwakura Izumi 岩倉　いずみ所属神奈川大学化学生命学部応用化学科神奈川大学大学院工学研究科工学専攻（応用化学領域）職種教授
言語種別	英語
発行・発表の年月	2008/01
形態種別	学術雑誌
査読	査読あり
標題	Synthesis of Staudinger-type Molecular Probe for Catch-and-release Purification of the Binding Protein for Potassium Isolespedezate.
執筆形態	共著
掲載誌名	Chemistry Letters,
掲載区分	国内
出版社・発行元	The Chemical Society of Japan
巻・号・頁	37(1),pp.52-53
著者・共著者	Tomohiko Fujii, Nobuki Kato, Izumi Iwakura, Yoshiyuki Manabe, Minoru Ueda
概要	We synthesized azide-containing photoaffinity probe 1 based on the structure of potassium isolespedezate. This probe can be used for catch-and-release-mechanism purification of binding protein for 1: photo-crosslinking with 1 gave azide-labeled receptor which can be captured by phosphane-linked gel matrix by the Staudinger ligation. After washing the gel, the caught binding protein can be released by the reductive cleavage of disulfide bond in 1. This process can be used as a convenient method for the purification of binding protein for bioactive natural product.
DOI	https://doi.org/10.1246/cl.2008.52
researchmap用URL	https://www.journal.csj.jp/doi/abs/10.1246/cl.2008.52