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イブカ アキコ
Ibuka Akiko 井深 章子 所属 神奈川大学 化学生命学部 生命機能学科 神奈川大学大学院 理学研究科 理学専攻(生物科学領域) 職種 教授 |
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言語種別 | 英語 |
発行・発表の年月 | 2007/12 |
形態種別 | 学術雑誌 |
査読 | 査読あり |
標題 | Molecular cloning and characterization of an alpha-amylase from Pichia burtonii 15-1 |
執筆形態 | 共著 |
掲載誌名 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY |
掲載区分 | 国外 |
出版社・発行元 | TAYLOR&FRANCIS LTD |
巻・号・頁 | 71(12),pp.3007-3013 |
著者・共著者 | Saemi Kato,Akiko Shimizu-Ibuka,Kiyoshi Mura,Akiko Takeuchi,Chiyoko Tokue,Soichi Arai |
概要 | An a-amylase secreted by Pichia burtonii 15-1 isolated from a traditional starter murcha of Nepal, named Pichia burtonii a-amylase (PBA), was studied. PBA was deduced to consist of 494 amino acid residues. It shared certain degrees of amino acid sequence identity with other homologous proteins: 60% with Schwanniomyces occidentalis alpha-amylase, 58% with Saccharomycopsis sp. alpha-amylase, and 47% with Takaamylase A from Aspergillus oryzae.Although the k(cat) values of PBA were lower than those of Taka-amylase A for the oligosaccharide substrates, the k(cat)/K-m values of PBA were higher. |
DOI | 10.1271/bbb.70407 |
ISSN | 09168451 |