イブカ アキコ   Ibuka Akiko
  井深 章子
   所属   神奈川大学  化学生命学部 生命機能学科
    神奈川大学大学院  理学研究科 理学専攻(生物科学領域)
   職種   教授
言語種別 英語
発行・発表の年月 2007/12
形態種別 学術雑誌
査読 査読あり
標題 Molecular cloning and characterization of an alpha-amylase from Pichia burtonii 15-1
執筆形態 共著
掲載誌名 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
掲載区分国外
出版社・発行元 TAYLOR&FRANCIS LTD
巻・号・頁 71(12),pp.3007-3013
著者・共著者 Saemi Kato,Akiko Shimizu-Ibuka,Kiyoshi Mura,Akiko Takeuchi,Chiyoko Tokue,Soichi Arai
概要 An a-amylase secreted by Pichia burtonii 15-1 isolated from a traditional starter murcha of Nepal, named Pichia burtonii a-amylase (PBA), was studied. PBA was deduced to consist of 494 amino acid residues. It shared certain degrees of amino acid sequence identity with other homologous proteins: 60% with Schwanniomyces occidentalis alpha-amylase, 58% with Saccharomycopsis sp. alpha-amylase, and 47% with Takaamylase A from Aspergillus oryzae.Although the k(cat) values of PBA were lower than those of Taka-amylase A for the oligosaccharide substrates, the k(cat)/K-m values of PBA were higher.
DOI 10.1271/bbb.70407
ISSN 09168451