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イブカ アキコ
Ibuka Akiko 井深 章子 所属 神奈川大学 化学生命学部 生命機能学科 神奈川大学大学院 理学研究科 理学専攻（生物科学領域） 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/09 |
| 形態種別 | 学術雑誌 |
| 査読 | 査読あり |
| 標題 | Structural and Mutagenic Analysis of Metallo-β-Lactamase IMP-18. |
| 執筆形態 | 共著 |
| 掲載誌名 | Antimicrobial agents and chemotherapy |
| 掲載区分 | 国外 |
| 巻・号・頁 | 60(9),pp.5521-5526 |
| 著者・共著者 | Furuyama Takamitsu,Nonomura Haruka,Ishii Yoshikazu,Hanson Nancy D,Shimizu-Ibuka Akiko |
| 概要 | The overall structure of IMP-18 resembles that of IMP-1, but the loop that covers the active site has a distinct conformation. The relationship between IMP-18's loop conformation and its kinetic properties was investigated by replacing the amino acid residues that can affect the loop conformation (Lys44, Thr50, and Ile69) in IMP-18 with those occupying the corresponding positions in the well-described enzyme IMP-1. The replacement of Thr50 with Pro considerably modified IMP-18's kinetic properties. The results indicate that this is a key residue that defines the kinetic properties of IMP-type enzymes. |
| DOI | 10.1128/AAC.00985-16 |
| ISSN | 10986596 |
| PMID | 27381398 |
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