研究者情報 | 神奈川大学

イノウエ　カズヒト Inoue Kazuhito 井上　和仁所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2010/10
形態種別	学術雑誌
査読	査読あり
標題	Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production
執筆形態	共著
掲載誌名	Applied and environmental microbiology
出版社・発行元	American Society for Microbiology
巻・号・頁	76(20),pp.6741-6750
著者・共著者	◎H. Masukawa, K. Inoue, H. Sakurai, C. P. Wolk, R. P. Hausinger
概要	In Anabaena sp. strain 7120, the enzyme is localized to the microaerobic environment of heterocysts, a highly differentiated subset of the filamentous cells. In an effort to increase H₂ production by this strain, six nitrogenase amino acid residues predicted to reside close to the FeMo-co were mutated. Most of the 49 variants examined were deficient in N₂-fixing growth and exhibited decreases in their in vivo rates of acetylene reduction. Of greater interest, several variants examined under an N₂ atmosphere significantly increased their in vivo rates of H₂ production, approximating rates equivalent to those under an Ar atmosphere, and accumulated high levels of H₂ compared to the reference strains.
DOI	10.1128/AEM.01056-10.