研究者情報 | 神奈川大学

イブカ　アキコ Ibuka Akiko 井深　章子所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2006/12
形態種別	学術雑誌
査読	査読あり
標題	Purification and characterization of an alpha-amylase of Pichia burtonii isolated from the traditional starter"murcha"in Nepal
執筆形態	共著
掲載誌名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
掲載区分	国外
出版社・発行元	TAYLOR&FRANCIS LTD
巻・号・頁	70(12),pp.3019-3024
著者・共著者	Akiko Takeuchi,Akiko Shimizu-Ibuka,Yoshitaka Nishiyama,Kiyoshi Mura,Sanae Okada,Chiyoko Tokue,Soichi Arai
概要	Among more than 20 yeast strains isolated from the traditional starter"murcha"in Nepal, we characterized a yeast that might be involved in saccharification. This strain, Pichia burtonii, produced an extracellular amylolytic enzyme when cultured in the presence of starch in the medium. We purified the enzyme and determined its N-terminal amino acid sequence. Together with the results of a hydrolyzing activity assay toward various substrates, it was found to be an a-amylase. This enzyme, Pichia burtonii a-amylase (PBA), was a glycoprotein with an apparent molecular mass of 51kDa. Enzyme activity was optimal at pH 5.0 at 40 degrees C. The enzyme retained 80% of its original activity after incubation under the optimal pH condition at 50 degrees C for 30 min. The activity was inhibited by metal ions such as Cd2+, Cu2+, Hg2+, Al3+, and Zn2+.
DOI	10.1271/bbb.60430
ISSN	09168451