研究者情報 | 神奈川大学

イブカ　アキコ Ibuka Akiko 井深　章子所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2006/08
形態種別	学術雑誌
査読	査読あり
標題	Effect of disulfide-bond introduction on the activity and stability of the extended-spectrum class A beta-lactamase Toho-1
執筆形態	共著
掲載誌名	BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
掲載区分	国外
出版社・発行元	ELSEVIER SCIENCE BV
巻・号・頁	1764(8),pp.1349-1355
著者・共著者	Akiko Shimizu-Ibuka,Hiroshi Matsuzawa,Hiroshi Sakai
概要	Both narrow spectrum and extended spectrum beta-lactamases of TEM and the SHV enzymes possess a disulfide bond between Cys77 and Cys123, and the enzymes with carbapenem-hydrolyzing activity have a well-conserved disulfide bridge between Cys69 and Cys238. We produced A77C/G123C mutant of the extended-spectrum beta-lactamase Toho-l in order to introduce a disulfide bond between the cysteine residues at positions 77 and 123. A new disulfide bridge was formed in the mutant, and it was indicated that the disulfide bridge stabilized the enzyme significantly. Though kinetic analysis indicated that the catalytic properties of the mutant were quite similar to those of the wild-type enzyme, E. coli producing this mutant showed drug resistance significantly higher than E. coli producing the wild-type enzyme.
DOI	10.1016/j.bbapap.2006.06.004
ISSN	15709639