研究者情報 | 神奈川大学

イブカ　アキコ Ibuka Akiko 井深　章子所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	1998
形態種別	学術雑誌
査読	査読あり
標題	Conversion of neopullulanase-α-amylase from Thermoactinomyces vulgaris R-47 into an amylopullulanase-type enzyme
執筆形態	共著
掲載誌名	Journal of Biochemistry
掲載区分	国外
出版社・発行元	Oxford University Press
巻・号・頁	123(2),pp.275-282
著者・共著者	Akiko Ibuka,Takashi Tonozuka,Hiroshi Matsuzawa,Hiroshi Sakai
概要	TVA I is a versatile enzyme which hydrolyzes the alpha-(1,4)-glucosidic linkages of pullulan to produce panose, known as neopullulanase activity, and the alpha-(1,6)-glucosidic linkages of certain oligosaccharides. We modified the Ala-357, Gln-359, and Tyr-360 residues located in region II, one of the four regions conserved in alpha-amylase family enzymes, and deleted 11 consecutive amino acid residues located after the C-terminus of region II of the TVA I sequence by means of site-directed mutagenesis. The action pattern of the mutated enzyme for pullulan was greatly altered and it hydrolyzed mainly the alpha-(1,6)-glucosidic linkages of pullulan to produce maltotriose, while the action patterns for starch and maltooligosaccharides were almost identical to those of the wild-type enzyme.
DOI	10.1093/oxfordjournals.jbchem.a021933
ISSN	0021924X
PMID	9538203