研究者情報 | 神奈川大学

イワクラ　イズミ Iwakura Izumi 岩倉　いずみ所属神奈川大学化学生命学部応用化学科神奈川大学大学院工学研究科工学専攻（応用化学領域）職種教授
言語種別	英語
発行・発表の年月	2013/01
形態種別	学術雑誌
査読	査読あり
標題	Triazoyl-Phenyl Linker System Enhancing the Aqueous Solubility of a Molecular Probe and its Efficiency in Affinity Labeling of a Target Protein for Jasmonate Glucoside.
執筆形態	共著
掲載誌名	Bioorganic & Medicinal Chemistry Letters
掲載区分	国外
出版社・発行元	ELSEVIER
巻・号・頁	23(1),pp.188-193
著者・共著者	Satoru Tamura, Sho Inomata, Makoto Ebine, Takahisa Genji, Izumi Iwakura, Makoto Mukai, Mitsuru Shoji, Takeshi Sugai, Minoru Ueda
概要	In methods employing molecular probes to explore the targets of bioactive small molecules, long or rigid linker moieties are thought to be critical factors for efficient tagging of target protein. We previously reported the synthesis of a jasmonate glucoside probe with a highly rigid linker consisting of a triazoylphenyl (TAzP) moiety, and this probe demonstrated effective target tagging. Here we compare the TAzP probe with other rigid or flexible probes with respect to target tagging efficiency, hydrophobic parameters, aqueous solubility, and dihedral angles around the biaryl linkage by a combination of empirical and calculation methods. The rigid biaryl linkage of the TAzP probe has a skewed conformation that influences its aqueous solubility. Such features that include rigidness and good aqueous solubility resulted in highly efficient target tagging. These findings provide a promising guideline toward designing of better linkers for improving molecular probe performance.
DOI	https://doi.org/10.1016/j.bmcl.2012.10.124
researchmap用URL	https://www.sciencedirect.com/science/article/pii/S0960894X12014266