研究者情報 | 神奈川大学

イブカ　アキコ Ibuka Akiko 井深　章子所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2010/09
形態種別	学術雑誌
査読	査読あり
標題	Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin
執筆形態	共著
掲載誌名	Biochimica et Biophysica Acta - General Subjects
掲載区分	国外
巻・号・頁	1800(9),pp.986-992
著者・共著者	Keisuke Ito,Taishi Sugawara,Ayako Koizumi,Ken-ichiro Nakajima,Akiko Shimizu-Ibuka,Mitsunori Shiroishi,Hidetsugu Asada,Takami Yurugi-Kobayashi,Tatsuro Shimamura,Tomiko Asakura,Katsuyoshi Masuda,Masaji Ishiguro,Takumi Misaka,So Iwata,Takuya Kobayashi,Keiko Abe
概要	A yeast expression system for MCL was constructed to accelerate analysis of its structure-function relationships. A 2. mg/l yield of rMCL was successfully obtained. Although rMCL was flat in taste under all the pH conditions employed, taste-modifying activity was recovered after deglycosylation. Mutagenetic analysis revealed that the N-glycan attached to Asn42 was bulky in rMCL.
DOI	10.1016/j.bbagen.2010.06.003
ISSN	03044165
PMID	20542090