イブカ アキコ   Ibuka Akiko
  井深 章子
   所属   神奈川大学  化学生命学部 生命機能学科
    神奈川大学大学院  理学研究科 理学専攻(生物科学領域)
   職種   教授
言語種別 英語
発行・発表の年月 2016/09
形態種別 学術雑誌
査読 査読あり
標題 Structural and Mutagenic Analysis of Metallo-β-Lactamase IMP-18.
執筆形態 共著
掲載誌名 Antimicrobial agents and chemotherapy
掲載区分国外
巻・号・頁 60(9),pp.5521-5526
著者・共著者 Furuyama Takamitsu,Nonomura Haruka,Ishii Yoshikazu,Hanson Nancy D,Shimizu-Ibuka Akiko
概要 The overall structure of IMP-18 resembles that of IMP-1, but the loop that covers the active site has a distinct conformation. The relationship between IMP-18's loop conformation and its kinetic properties was investigated by replacing the amino acid residues that can affect the loop conformation (Lys44, Thr50, and Ile69) in IMP-18 with those occupying the corresponding positions in the well-described enzyme IMP-1. The replacement of Thr50 with Pro considerably modified IMP-18's kinetic properties. The results indicate that this is a key residue that defines the kinetic properties of IMP-type enzymes.
DOI 10.1128/AAC.00985-16
ISSN 10986596
PMID 27381398