研究者情報 | 神奈川大学

イブカ　アキコ Ibuka Akiko 井深　章子所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2004/12
形態種別	学術雑誌
査読	査読あり
標題	An engineered disulfide bond between residues 69 and 238 in extended-spectrum beta-lactamase Toho-1 reduces its activity toward third-generation cephalosporins
執筆形態	共著
掲載誌名	BIOCHEMISTRY
掲載区分	国外
出版社・発行元	AMER CHEMICAL SOC
巻・号・頁	43(50),pp.15737-15745
著者・共著者	A Shimizu-Ibuka, H Matsuzawa, H Sakai
概要	Previous crystallographic structural analysis of Toho-1 predicted that the high flexibility of beta-strand B3 could be one of the key features contributing to Toho-1 activity toward third-generation cephalosporins. Here we constructed two Toho-1 mutants, G238C and G238C/G239in, to introduce a disuffide bond between the cysteine residues at positions 69 and 238, and thus to lock the position of beta-strand B3. A new disulfide bridge was formed in the G238C mutant. Kinetic analysis showed that the activity of the G238C mutant decreased drastically against third-generation cephalosporins.
DOI	10.1021/bi048488u
ISSN	00062960