研究者情報 | 神奈川大学

イブカ　アキコ Ibuka Akiko 井深　章子所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2003/09
形態種別	学術雑誌
査読	査読あり
標題	Crystal structure of extended-spectrum beta-lactamase Toho-1: Insights into the molecular mechanism for catalytic reaction and substrate specificity expansion
執筆形態	共著
掲載誌名	BIOCHEMISTRY
掲載区分	国外
出版社・発行元	AMER CHEMICAL SOC
巻・号・頁	42(36),pp.10634-10643
著者・共著者	AS Ibuka, Y Ishii, M Galleni, M Ishiguro, K Yamaguchi, JM Frere, H Matsuzawa, H Sakai
概要	The crystallographic structure of the class A beta-lactamase Toho-1, an extended-spectrum beta-lactamase with potent activity against expanded-spectrum cephems, has been determined at 1.65 Angstrom resolution. The result reveals that the Lys73 side chain can adopt two alternative conformations. The Lys73 side chain would play an important role in proton relay, switching its conformation from one to the other depending on the circumstances. The electron density map also implies possible rotation of Ser237. Comparison of the Toho-1 structure with the structure of other class A beta-lactamases shows that the hydroxyl group of Ser237 is likely to rotate through interaction with the carboxyl group of the substrate.
DOI	10.1021/bi0342822
ISSN	00062960