イノウエ カズヒト   Inoue Kazuhito
  井上 和仁
   所属   神奈川大学  理学部 生物科学科
   職種   教授
言語種別 英語
発行・発表の年月 2008/04
形態種別 学術雑誌
査読 査読あり
標題 NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters.
執筆形態 共著
掲載誌名 FEBS Letters
巻・号・頁 582,pp.1346-1350
著者・共著者 Nomata J., Ogawa T., Kitashima M., Inoue K. and Fujita Y
概要 Dark operative protochlorophyllide (Pchlide) oxidoreductase is a nitrogenase like enzyme consisting of the two components, Lprotein (BchL-dimer) and NB-protein (BchN BchB heterotetramer). Here, we show that NBprotein is the catalytic component with FeS clusters. NB protein purified from Rhodobacter capsulatus bound Pchlide that was readily converted to chlorophyllide a upon the addition of Lprotein and MgATP. The activity of NB protein was resistant to the exposure to air. A Pchlide free form of NB-protein purified from a bchH-lacking mutant showed an absorption spectrum suggesting the presence of FeS centers. Together with the Fe and sulfide contents, these findings suggested that NB-protein carries two oxygen-tolerant [4Fe4S] clusters.
DOI 10.1016/j.febslet.2008.03.018