研究者情報 | 神奈川大学

イノウエ　カズヒト Inoue Kazuhito 井上　和仁所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2008/04
形態種別	学術雑誌
査読	査読あり
標題	NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters.
執筆形態	共著
掲載誌名	FEBS Letters
巻・号・頁	582,pp.1346-1350
著者・共著者	Nomata J., Ogawa T., Kitashima M., Inoue K. and Fujita Y
概要	Dark operative protochlorophyllide (Pchlide) oxidoreductase is a nitrogenase like enzyme consisting of the two components, Lprotein (BchL-dimer) and NB-protein (BchN BchB heterotetramer). Here, we show that NBprotein is the catalytic component with FeS clusters. NB protein purified from Rhodobacter capsulatus bound Pchlide that was readily converted to chlorophyllide a upon the addition of Lprotein and MgATP. The activity of NB protein was resistant to the exposure to air. A Pchlide free form of NB-protein purified from a bchH-lacking mutant showed an absorption spectrum suggesting the presence of FeS centers. Together with the Fe and sulfide contents, these findings suggested that NB-protein carries two oxygen-tolerant [4Fe4S] clusters.
DOI	10.1016/j.febslet.2008.03.018