研究者情報 | 神奈川大学

イノウエ　カズヒト Inoue Kazuhito 井上　和仁所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2009
形態種別	学術雑誌
査読	査読あり
標題	Survey of the distribution of different types of nitrogenases and hydrogenases in heterocyst-forming cyanobacteria
執筆形態	共著
掲載誌名	Marine Biotechnology.
出版社・発行元	Springer
巻・号・頁	11,pp.397-409
著者・共著者	H. Masukawa, X. Zhang, Emi Yamazaki, Syunsuke Iwata, Kensuke Nakamura, Mari Mochimaru, Kazuhito Inoue, Hidehiro Sakurai
概要	We surveyed the distribution of these genes and their activities in 14 strains from culture collections. The nitrogenase genes include nif1 encoding a Mo-type nitrogenase expressed in heterocysts, nif2 expressed in vegetative cells and heterocysts under anaerobic conditions, and vnf encoding a V-type nitrogenase expressed in heterocysts. We found that all 14 strains have nifD1 genes, and eight strains also have nifD2 genes. Four of the strains have vnfD genes, in addition to nifD2 genes. For surveying the distribution of hydrogenases, Southern blot hybridization was an effective method. All strains surveyed had hup genes, with the majority of them also having hox genes.
DOI	10.1007/s10126-008-9156-z