イノウエ カズヒト   Inoue Kazuhito
  井上 和仁
   所属   神奈川大学  理学部 生物科学科
   職種   教授
言語種別 英語
発行・発表の年月 2008/09
形態種別 学術雑誌
査読 査読あり
標題 SoxAX binding protein, a novel component of the thiosulfate-oxidizing multienzyme system in the green sulfur bacterium Chlorobium tepidum
執筆形態 共著
掲載誌名 J. Bacteriol.
出版社・発行元 American society for Microbiology
巻・号・頁 190,pp.6097-6110
著者・共著者 ◎T. Ogawa, T. Furusawa,R. Nomura, D. Seo, N. M. Matsuda, H. Sakurai, K. Inoue
概要 From the photosynthetic green sulfur bacterium Chlorobium tepidum , we have purified three factors indispensable for the thiosulfate-dependent reduction of the small, monoheme cytochrome c554. The first factor is SoxYZ , the second factor is monomeric SoxB and the third factor is the trimeric cytochrome c551, composed of the monoheme cytochrome SoxA, the monoheme cytochrome SoxX, and the product of the hypothetical open reading frame CT1020. In the presence of the other two Sox factors, the recombinant SoxA and SoxX showed a low but discernible thiosulfate-dependent cytochrome c554 reduction activity. The further addition of the recombinant CT1020 protein greatly increased the activity, and the total activity was as high as that of the native SoxAX-CT1020 protein complex. The recombinant CT1020 protein participated in the formation of a tight complex with SoxA and SoxX and will be referred to as SAXB (SoxAX binding protein).
DOI 10.1128/JB.00634-08