イノウエ カズヒト   Inoue Kazuhito
  井上 和仁
   所属   神奈川大学  理学部 生物科学科
   職種   教授
言語種別 英語
発行・発表の年月 2010/10
形態種別 学術雑誌
査読 査読あり
標題 Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production
執筆形態 共著
掲載誌名 Applied and environmental microbiology
出版社・発行元 American Society for Microbiology
巻・号・頁 76(20),pp.6741-6750
著者・共著者 ◎H. Masukawa, K. Inoue, H. Sakurai, C. P. Wolk, R. P. Hausinger
概要 In Anabaena sp. strain 7120, the enzyme is localized to the microaerobic environment of heterocysts, a highly differentiated subset of the filamentous cells. In an effort to increase H2 production by this strain, six nitrogenase amino acid residues predicted to reside close to the FeMo-co were mutated. Most of the 49 variants examined were deficient in N2-fixing growth and exhibited decreases in their in vivo rates of acetylene reduction. Of greater interest, several variants examined under an N2 atmosphere significantly increased their in vivo rates of H2 production, approximating rates equivalent to those under an Ar atmosphere, and accumulated high levels of H2 compared to the reference strains.
DOI 10.1128/AEM.01056-10.