イノウエ カズヒト   Inoue Kazuhito
  井上 和仁
   所属   神奈川大学  理学部 生物科学科
   職種   教授
言語種別 英語
発行・発表の年月 2014/11
形態種別 学術雑誌
査読 査読あり
標題 Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena
執筆形態 共著
掲載誌名 International Journal of Hydrogen Energy
出版社・発行元 Springer
巻・号・頁 39,pp.19444-19451
著者・共著者 ◎H. Masukawa, H. Sakurai, R. P. Hausinger, K. Inoue
概要 Two double-crossover variants were constructed with amino acid substitutions in nitrogenase. The variants continuously produced H2 over 21 days under N2-rich atmosphere. Extremely low N2-fixing activity of the variant nitrogenases allowed cells to obtain combined N to sustain their activity. The dc-Q193S variant produced ethane at low but significant rates along with greater rates of ethylene production. Sustained H2 production by the Anabaena ΔHup cells can be attained under low N2 concentration in the headspace gases.
DOI 10.1016/j.ijhydene.2014.09.090