研究者情報 | 神奈川大学

イノウエ　カズヒト Inoue Kazuhito 井上　和仁所属神奈川大学化学生命学部生命機能学科神奈川大学大学院理学研究科理学専攻（生物科学領域）職種教授
言語種別	英語
発行・発表の年月	2014/11
形態種別	学術雑誌
査読	査読あり
標題	Sustained photobiological hydrogen production in the presence of N₂ by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena
執筆形態	共著
掲載誌名	International Journal of Hydrogen Energy
出版社・発行元	Springer
巻・号・頁	39,pp.19444-19451
著者・共著者	Hajime Masukawa, Hidehiro Sakurai, Robert P. Hausinger, Kazuhito Inoue
概要	Two double-crossover variants were constructed with amino acid substitutions in nitrogenase. The variants continuously produced H2 over 21 days under N2-rich atmosphere. Extremely low N₂-fixing activity of the variant nitrogenases allowed cells to obtain combined N to sustain their activity. The dc-Q193S variant produced ethane at low but significant rates along with greater rates of ethylene production. Sustained H2 production by the Anabaena ΔHup cells can be attained under low N₂ concentration in the headspace gases.
DOI	10.1016/j.ijhydene.2014.09.090