イノウエ カズヒト   Inoue Kazuhito
  井上 和仁
   所属   神奈川大学  理学部 生物科学科
   職種   教授
言語種別 英語
発行・発表の年月 2015/10
形態種別 学術雑誌
査読 査読あり
標題 Involvement of thioredoxin on the scaffold activity of NifU in heterocyst cells of the diazotrophic cyanobacterium Anabaena sp. strain PCC 7120
執筆形態 共著
掲載誌名 Journal of Biochemistry
掲載区分国外
巻・号・頁 158(3),pp.253-261
著者・共著者 Jiro Nomata, Maki Maeda, Atsuko Isu, Kazuhito Inoue, Toru Hisabori
概要 The diazotrophic cyanobacterium Anabaena sp. strain PCC 7120 differentiates into specialized heterocyst cells that fix nitrogen under nitrogen starvation conditions. Although reducing equivalents are essential for nitrogen fixation, little is known about redox systems in heterocyst cells. In this study, we investigated thioredoxin (Trx) networks in Anabaena using TrxM, and identified 16 and 38 candidate target proteins in heterocysts and vegetative cells, respectively, by Trx affinity chromatography. Among these, the FeS cluster scaffold protein NifU that facilitates functional expression of nitrogenase in heterocysts was found to be a potential TrxM target. Subsequently, we observed that the scaffold activity of N-terminal catalytic domain of NifU is enhanced in the presence of Trx-system, suggesting that TrxM is involved in the FeS cluster biogenesis.
DOI 10.1093/jb/mvv046